anti itgb1  (Cell Signaling Technology Inc)

Journal: Materials Today Bio

Article Title: Uniform and controllable surface nano-structure on polyetheretherketone implants can regulate mechanical property to enhance soft tissue integration through Piezo1/TGF-β1 signaling axis

doi: 10.1016/j.mtbio.2025.101645

Figure Lengend Snippet: The impact of nanoscale modification of PEEK implant surfaces on the mechanical perception, proliferation, and expression of fibrosis-related proteins in L929 cells. (A) The expression levels of mechanical perception, proliferation, and fibrosis-related proteins were evaluated in L929 cells cultured on different nanoscale modified PEEK surfaces at multiple time points. (B) The secretion of TGF-β1 from L929 cells cultured on various nanoparticle-modified PEEK surfaces was quantified using Elisa at multiple time points. (C) Immunofluorescence analysis was conducted to assess the expression differences of Piezo1, ITGB1, and α-SMA in L929 cells cultured on different nanoparticle-modified PEEK surfaces. (D) Quantitative fluorescence analysis was performed on the data presented in C.

Article Snippet: The primary antibodies targeting Piezo1 (A23380, rabbit) and Fibronectin (A25907, rabbit) were acquired from Cell Signaling Technology, Inc. ITGB1 (34971T, rabbit) was procured from Cell Signaling Technology, Inc. TGF-β1 (21898-1-AP), α-SMA (14395-1-AP), and YAP1 (13584-1-AP) were acquired from Proteintech Group, Inc. Smad2/3 (sc-133098) was acquired from Santa Cruz Biotechnology, Inc. IL-6 (GB11117) and TNF-α (GB115702) was acquired from Wuhan Servicebio Technology Co., Ltd., China.

Techniques: Modification, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Fluorescence